人类和机械生命体决战后过了1000年，地球逐渐从颓废中恢复了过来，但是兵强马壮的巴西帝国又发动了征服世界的战争。 少女伊可儿偶然遇到了1000年前大战中幸存的机动兵器“夏安”，一人一机开始了冒险之旅……
In the year 1524, the former Assassin Ezio Auditore retired to a villa in the countryside of Tuscany, with his wife Sofia and children Marcello and Flavia. While Ezio wrote in his study, Sofia asked him to watch Flavia while she and Marcello visit his sister Claudia. Shortly after, he left his study to tend their vineyards while Flavia played nearby the villa. In the midst of his harvest, he saw a stranger cloaked in black, talking to Flavia. Fearing the worst, Ezio hurriedly intercepted the stranger, though Flavia told him that she was no danger, as Ezio saw her necklace with the Assassin insignia...
